Trung Son T.S.S.E Scientific Equipment & Tourist Joint Stock Company

Joint Stock Company

Trung Son T.S.S.E Scientific Equipment & Tourist Joint Stock Company is an Exclusive Distributor of SHIMADZU, a famous brand of analytical instruments, since 2000 in Viet Nam

Of the short-chain fatty acids produced by intestinal bacteria, acetic acid, propionic acid, and butyric acid are well known, and it has been reported that there are some connections between them and lifestyle-related diseases such as obesity and diabetes. Generally speaking, short-chain fatty acids are highly volatile and highly hydrophilic. This makes it difficult to perform LCMS analysis using a conventional reversed phase system. For that reason, this method package targets short-chain fatty acids (C2 to C5) that have been derivatized using 3-nitrophenylhydrazine (3-NPH). After setting MRM transitions, it can be used for the simultaneous analysis of 22 components, including organic acids related to the central metabolic pathways

Short-Chain Fatty Acids Organic Acids
Acetic acid 2-oxobutyric acid
Propionic acid 2-hydroxyglutaric acid
Butyric acid α-ketoglutaric acid
Isobutyric acid β-hydroxybutyric acid
Valeric acid Isocitric acid
Isovaleric acid Oxaloacetic acid
  Citric acid
  Glyoxylic acid
  Glycolic acid
  Succinic acid
  Lactic acid
  Pyruvic acid
  Fumaric acid
  Maleic acid
  Malonic acid
  Malic acid
Pretreatment Protocol Including Derivatization
The entire protocol necessary for pretreatment, including the derivatization step using 3-nitrophenylhydrazine, is contained in the Instruction Manual. By following these procedures, an analyst should be able to perform the entire process, from derivatization to data acquisition and data analysis, immediately after the package is introduced.