Shimadzu Việt Nam - Trung Son T.S.S.E Scientific Equipment & Tourist Joint Stock Company

Joint Stock Company

Shimadzu Vietnam - Trung Son T.S.S.E Scientific Equipment & Tourist Joint Stock Company is an Exclusive Distributor of SHIMADZU, a famous brand of analytical instruments, since 2000 in Viet Nam

Remdesivir (brand name: Veklury®), which was developed by Gilead Science (U.S.) for treatment of Ebola virus disease, is a prodrug having antiviral activity against single-strand RNA viruses. It is known to be partly metabolized to activated GS-441524, the main metabolite of remdesivir, in vivo1). In this article, we present the results of research into an analytical system that can analyze remdesivir and its metabolites simultaneously using LC/MS/MS, an analytical method that demonstrates outstanding selectivity

Remdesivir (P/N: C8799*) and GS-441524 (P/N: C8847*), as the target compounds, and [U-Ring-13C6]-remdesivir (P/N: C8845*) and [13C5]-GS-441524 (P/N: C8855*), as their stable isotopes, were purchased from Alsachim, one of the companies of the Shimadzu Group. [U-Ring-13C6]-remdesivir and [13C5]-GS441524 were used as materials of the internal standard. To commercially available human plasma treated with EDTA 2K, remdesivir and GS-441524 were added. Following this, the calibration curves were prepared. Shim-pack ScepterTM C18-120 (50 mm×2.1 mm I.D., 1.9 μm) was used as the analytical column.

Calibration was performed using 5 calibration points at concentrations of 100, 500, 1000, 2500 and 5000 ng/mL for remdesivir and 5 calibration points at concentrations of 5, 25, 50, 250 and 500 ng/mL for GS-441524 (n = 5 for each calibration point). [U-Ring-13C6]-remdesivir (2.5 μg/mL) and [13C5]-GS441524 (0.25 μg/mL) were mixed with methanol to be used as the internal standard (ISTD).

The samples are prepared in the following sequence. After 20μL of 75% IPA, 50 μL of plasma, 10 μL of ISTD and 100 μL of acetonitrile were added and mixed well, the mixture was centrifuged. The supernatant obtained by centrifuging was transferred into an LC vial for analysis.